Structural MS approaches (Top-down, HDX-MS) are highly complementary to proteomics to characterize Proteasome heterogeneity, dynamics, Post-Translational Modifications (PTMs), and interaction interfaces.
We have optimized them to work on poorly concentrated and very heterogeneous proteasome complexes. New important mechanistic insights in proteasome regulation could be highlighted (Lesne et al. Bioinformatics and biology insights 2019; Lesne et al. Nature Commun. 2020).