We have developed a quantitative MS-based targeted MRM (Multiple Reaction Monitoring) approach to measure the quantity of 20S proteasome subtypes in biological samples (Menneteau et al. Mol. Cell. Proteomics.).
In collaboration with I. Ader (EFS-Stromalab, Toulouse), we have shown O2-dependent changes in 20S composition during expansion of adipose-derived stem cells (ADSCs) used for cellular therapies.