Interactomics: complexes that assemble around different nodes of the TCR signaling cascade in primary CD4+ T cells

Interactomics: complexes that assemble around different nodes of the TCR signaling cascade in primary CD4+ T cells

We analyze by mass spectrometry signalling complexes that form around canonical proteins used by the proximal TCR signal transduction pathway, and monitor their dynamic of assembly in the first minutes following engagement of the TCR.

In collaboration with B. Malissen and R. Roncagalli (CIML, Marseille), we use KI mouse model expressing different bait proteins of the TCR pathway with a C-terminal One-Strep-tag to enrich endogenous signaling complexes at different time points following TCR activation. Time resolved proteomics provides dynamics information (see for example the formation of the VAV1 interactome) and insights into the mechanisms of action of several proteins.

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